E ffects of chronic ethanol consumption on rat GABA and strychnine - A sensitive glycine receptors expressed by lateral / basolateral amygdala neurons 1 * Brian

It is well known that the anxiolytic potential of ethanol is maintained during chronic exposure. We have confirmed this using a light–dark box paradigm following chronic ethanol ingestion via a liquid diet. However, cessation from chronic ethanol exposure is known to cause severe withdrawal anxiety. These opposing effects on anxiety likely result from neuro-adaptations of neurotransmitter systems within the brain regions regulating anxiety. Recent work highlights the importance of amygdala ligand-gated chloride channels in the expression of anxiety. We have therefore examined the effects of chronic ethanol exposure on GABA and strychnine-sensitive A glycine receptors expressed by acutely isolated adult rat lateral /basolateral amygdala neurons. Chronic ethanol exposure increased the functional expression of GABA receptors in acutely isolated basolateral amygdala neurons without altering strychnine-sensitive glycine A receptors. Neither the acute ethanol nor benzodiazepine sensitivity of either receptor system was affected. We explored the likelihood that subunit composition might influence each receptor’s response to chronic ethanol. Importantly, when expressed in a mammalian heterologous system, GABA receptors composed of unique a subunits were differentially sensitive to acute ethanol. Likewise, the A presence of the b subunit appeared to influence the acute ethanol sensitivity of glycine receptors containing the a subunit. Our results 2 suggest that the facilitation of GABA receptors during chronic ethanol exposure may help explain the maintenance of ethanol’s A anti-anxiety effects during chronic ethanol exposure. Furthermore, the subunit composition of GABA and strychnine-sensitive glycine A receptors may ultimately influence the response of each system to chronic ethanol exposure.  2002 Elsevier Science B.V. All rights reserved. Theme: Neural basis of behaviour Topic: Drugs of abuse: alcohol, barbiturates, and benzodiazepines